DNA

Part:BBa_K4887006:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-08-14)


HygR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Hygromycin resistance gene is used to select the positive transformants when the transformed cells are cultured on media with hygromycin.


Source

Streptomyces hygroscopicus.


References